Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gamma radiation induces CD69 expression on lymphocytes

J C Chen1, B H Davis, M A Leon

  • 1Department of Clinical Pathology, William Beaumont Hospital, Royal Oak, Michigan 48073-6769, USA. jchen@smtpgw.beaumont.edu

Cytometry
|January 24, 1998
PubMed
Summary

Gamma radiation increases CD69 expression on T and B cells, acting as a radiation response marker. However, this radiation-induced CD69 expression does not stimulate lymphocyte proliferation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fischer-Tropsch synthesis. Evaluation of an aluminum small channel reactor.

Faraday discussions·2017
Same author

Technical Report: Triple-Colour Staining Flow Cytometry for Co-Distribution of Thrombospondin Receptor (CD36), Ribonucleic Acid (RNA) and Fetal Haemoglobin (HbF) in Sickle Red Blood Cells.

Hematology (Amsterdam, Netherlands)·2016
Same author

The challenge of tetradic relationships in medically interpreted pediatric primary care visits: A descriptive study of communication practices.

Patient education and counseling·2016
Same author

Use of CD157 in FLAER-based assays for high-sensitivity PNH granulocyte and PNH monocyte detection.

Cytometry. Part B, Clinical cytometry·2013
Same author

Use of CD157 in FLAER-based assays for high-sensitivity PNH granulocyte and PNH monocyte detection.

Cytometry. Part B, Clinical cytometry·2013
Same author

Automated quantitation of fetomaternal hemorrhage by flow cytometry for HbF-containing fetal red blood cells using probability state modeling.

International journal of laboratory hematology·2013

Area of Science:

  • Immunology
  • Radiation Biology
  • Cell Signaling

Background:

  • Gamma radiation is known to activate protooncogenes involved in signal transduction pathways.
  • While gene expression changes in lymphocytes post-gamma radiation are studied, early surface receptor expression remains underreported.
  • Investigating early cellular responses to radiation is crucial for understanding lymphocyte behavior.

Purpose of the Study:

  • To investigate the effect of gamma radiation on lymphocyte CD69 surface receptor expression.
  • To assess the impact of radiation on lymphocyte proliferation, measured by BrdU uptake.
  • To examine these effects in the presence and absence of phytohemagglutinin (PHA).

Main Methods:

  • Lymphocytes were exposed to varying doses of gamma radiation.

Related Experiment Videos

  • CD69 expression was measured using flow cytometry.
  • Cell proliferation was assessed by bromodeoxyuridine (BrdU) incorporation.
  • Experiments were conducted with and without phytohemagglutinin (PHA) stimulation.

    • Main Results:

    • Gamma radiation induced CD69 expression on both T and B lymphocytes in a dose- and time-dependent manner.
    • Peak CD69 expression occurred at 4 hours post-irradiation (906 cGy), with ~90% of B cells and ~12% of T cells affected.
    • Radiation alone did not stimulate BrdU incorporation, indicating no proliferation.
    • Radiation enhanced PHA-induced CD69 expression but inhibited PHA-induced BrdU incorporation at later time points.

    Conclusions:

    • CD69 serves as an early marker for lymphocyte response to gamma radiation.
    • Radiation-induced CD69 expression on lymphocytes does not lead to proliferation, differentiating it from antigen or mitogen responses.
    • Gamma radiation modulates lymphocyte activation markers and proliferation pathways independently.