Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Electron tomography of large, multicomponent biological structures

G A Perkins1, C W Renken, J Y Song

  • 1Department of Biology, San Diego State University, California 92182-4614, USA.

Journal of Structural Biology
|January 27, 1998
PubMed
Summary

Electron tomography reconstructs 3D structures from microscope images. This study details its use for complex biological samples like mitochondria, including computational tools and efficient reconstruction methods.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Association between light exposure at night and pubertal development in children and adolescents].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2026
Same author

[Comparison of outcomes between emergency surgery and conservative treatment for acute type A aortic intramural hematoma].

Zhonghua yi xue za zhi·2026
Same author

[Microimplant-assisted rapid palatal expansion treatment of a case of skeletal class Ⅲ malocclusion].

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology·2025
Same author

[Clinicopathological features of hyalinizing clear cell carcinoma of the salivary glands: an analysis of 65 cases].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2025
Same author

[Therapeutic application of cytokine antagonists in chronic obstructive pulmonary disease].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2025
Same author

[Development and validation of the rapid health aging assessment scale for the Chinese population].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2025

Area of Science:

  • Cell Biology
  • Structural Biology
  • Biophysics

Background:

  • Electron tomography (ET) is crucial for determining 3D structures from electron microscopy (EM) data.
  • Reconstructing large, complex biological specimens presents significant challenges.

Purpose of the Study:

  • To describe the application of electron tomography for reconstructing large, complex cellular structures.
  • To present tools for analyzing tomographic data.
  • To discuss efficient computational methods for tomographic reconstruction.

Main Methods:

  • Electron tomography (ET) applied to biological samples.
  • Development and integration of segmentation, measurement, classification, and visualization tools.
  • Utilizing massively parallel computers for R-weighted backprojection and iterative reconstruction algorithms.

Related Experiment Videos

Main Results:

  • Demonstrated successful 3D reconstruction of complex structures like mitochondria using ET.
  • Integrated software tools facilitate comprehensive analysis of tomographic data.
  • Efficient reconstruction achieved through parallel computing techniques.

Conclusions:

  • Electron tomography is a powerful technique for high-resolution 3D structural analysis of cellular components.
  • The described computational tools and methods enhance the efficiency and scope of ET applications.
  • This approach enables detailed structural insights into organelles such as mitochondria.