Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hemoglobin typing by high performance liquid chromatography

T Tatu1, P Gategasem, P Hathirat

  • 1Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.

The Southeast Asian Journal of Tropical Medicine and Public Health
|June 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Disarticulation of a knee joint in a haemophiliac with high inhibitor titre.

Haemophilia : the official journal of the World Federation of Hemophilia·2016
Same author

A novel test tube method of screening for hemoglobin E.

International journal of laboratory hematology·2011
Same author

Survival analysis of patients with haemophilia at the International Haemophilia Training Centre, Bangkok, Thailand.

Haemophilia : the official journal of the World Federation of Hemophilia·2004
Same author

Full chimerism in nonmyeloablative stem cell transplantation in a beta-thalassemia major patient (class 3 Lucarelli).

Bone marrow transplantation·2002
Same author

Thromboembolic complications in Thai pediatric patients.

Journal of the Medical Association of Thailand = Chotmaihet thangphaet·2001
Same author

HLA alloimmunization in patients receiving multitransfusions of red blood cells.

The Southeast Asian journal of tropical medicine and public health·2001

High performance liquid chromatography (HPLC) shows promise for hemoglobin typing, accurately measuring Hb E and correlating well for Hb A2. It is a viable alternative for hemoglobin analysis, especially for Hb F levels above 2.0%.

Area of Science:

  • Clinical Chemistry
  • Hematology
  • Analytical Chemistry

Background:

  • Hemoglobin typing is crucial for diagnosing hemoglobinopathies.
  • Routine methods for hemoglobin analysis include electrophoresis and chromatography.
  • High performance liquid chromatography (HPLC) offers a potential alternative for hemoglobin typing.

Purpose of the Study:

  • To evaluate the efficacy of HPLC for hemoglobin typing.
  • To compare HPLC results with established methods for Hb A2, Hb E, and Hb F quantification.
  • To determine the potential of HPLC as an alternative diagnostic tool.

Main Methods:

  • Blood specimens were analyzed using HPLC and compared with routine methods.
  • Quantification of Hb A2, Hb E, and Hb F levels was performed.

Related Experiment Videos

  • Statistical analysis was employed to compare results from different techniques.

    • Main Results:

    • HPLC showed good correlation with electrophoresis and microcolumn chromatography for Hb A2, despite statistical differences.
    • HPLC demonstrated very good correlation with electrophoresis for Hb E.
    • HPLC correlated well with the Betke alkaline denaturation test for Hb F when levels exceeded 2.0%.

    Conclusions:

    • HPLC is a potential alternative method for hemoglobin typing.
    • The accuracy of HPLC for Hb F measurement is reliable for levels above 2.0%.
    • An equation is provided for calculating alkaline denaturation Hb F from HPLC data for levels ≥10%.