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Preventive effect of dapsone on renal scarring following mannose-sensitive piliated bacterial infection
O Mochida1, T Matsumoto, Y Mizunoe
1Department of Urology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Abstract:
Renal scarring has been thought to occur in the later stages of chronic pyelonephritis. We previously reported that mannose-sensitive (MS) piliated bacteria promoted renal scarring, which was prevented by antioxidants. The preventive effect of diaphenylsulfone (dapsone), which has a scavenging activity on active oxygen species, on renal scarring was examined. Female Sprague-Dawley rats were inoculated with clinical isolates of Serratia marcescens which had both MS and mannose-resistant pili or with recombinant strains which had MS pili on their surface; they were then administered 20 mg/kg of dapsone or not. Dapsone significantly suppressed scarring following infection of the kidney. The bacterial counts in the kidneys were not different in dapsone-treated and nontreated rats. We conclude that dapsone is effective in preventing renal scarring, and it is suggested that the clinical use of this drug may prevent renal scar formation following pyelonephritis.
Insights
Dapsone, an antioxidant, effectively prevents kidney scarring caused by pyelonephritis. This drug reduces scarring without affecting bacterial load, suggesting its potential for clinical use in preventing renal damage.
Area of Science:
- Nephrology
- Microbiology
- Pharmacology
Background:
- Renal scarring is a known complication of chronic pyelonephritis.
- Mannose-sensitive (MS) piliated bacteria and reactive oxygen species contribute to renal scarring.
- Antioxidants have previously shown promise in preventing this scarring.
Purpose of the Study:
- To investigate the preventive effect of dapsone, an antioxidant, on renal scarring induced by pyelonephritis.
- To determine if dapsone impacts bacterial load in the kidneys during infection.
Main Methods:
- Female Sprague-Dawley rats were infected with Serratia marcescens strains possessing MS pili.
- Rats received either 20 mg/kg of dapsone or no treatment.
- Renal scarring and bacterial counts in the kidneys were assessed.
Main Results:
- Dapsone significantly suppressed the development of renal scarring.
- No significant difference in bacterial counts was observed between dapsone-treated and untreated rats.
- This indicates dapsone's protective effect is independent of bacterial clearance.
Conclusions:
- Dapsone is effective in preventing renal scar formation following pyelonephritis.
- The findings suggest dapsone's potential clinical utility in managing or preventing kidney damage associated with pyelonephritis.
- Dapsone's antioxidant properties appear crucial for mitigating renal scarring.