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Related Experiment Videos

DNA immunization against herpes simplex virus: enhanced efficacy using a Sindbis virus-based vector

M J Hariharan1, D A Driver, K Townsend

  • 1Department of Viral Therapeutics, Center for Gene Therapy, Chiron Technologies, San Diego, California 92121-1204, USA.

Journal of Virology
|January 28, 1998
PubMed
Summary

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Sindbis virus-based pSIN vectors effectively induce robust immune responses for DNA vaccines. These alphavirus vectors show superior efficacy and require lower doses compared to conventional plasmid DNA vaccines, demonstrating potential for herpes simplex virus vaccines.

Area of Science:

  • Virology
  • Immunology
  • Vaccinology

Background:

  • Alphavirus-based vectors, specifically Sindbis virus (SIN)-derived pSIN vectors, enable high-level gene expression through self-amplifying RNA replication in vitro.
  • Previous research established the foundation for these SIN-derived vectors.

Purpose of the Study:

  • To evaluate the in vivo efficacy of pSIN vectors as DNA vaccines.
  • To compare the immunogenicity of pSIN vectors against conventional plasmid DNA vectors.

Main Methods:

  • BALB/c mice were immunized intramuscularly with pSIN vectors encoding herpes simplex virus type 1 glycoprotein B.
  • Immune responses, including antibody production, cytotoxic T cell activity, and protection against viral challenge, were assessed.
  • Dosing studies compared pSIN vectors with conventional DNA vectors.

Related Experiment Videos

Main Results:

  • A single intramuscular immunization with pSIN vectors induced comprehensive immune responses, including virus-specific antibodies and cytotoxic T cells.
  • pSIN vectors conferred protection against lethal herpes simplex virus challenge in two murine models.
  • Dosing studies revealed pSIN vectors were 100- to 1,000-fold more potent than conventional DNA vectors, with significant responses seen at doses as low as 10 ng.

Conclusions:

  • pSIN vectors demonstrate significant potential as effective DNA vaccines.
  • Alphavirus-based vectors offer a promising platform for DNA immunization, particularly for vaccines against herpes simplex virus.