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Related Experiment Videos

mRNA instability elements in the human papillomavirus type 16 L2 coding region

M Sokolowski1, W Tan, M Jellne

  • 1Microbiology and Tumorbiology Center, Karolinska Institute, Stockholm, Sweden.

Journal of Virology
|January 28, 1998
PubMed
Summary

Human papillomavirus type 16 L2 gene has inhibitory sequences that block protein production in dividing cells. These sequences may help the virus evade immune detection and establish persistent infections.

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Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Human papillomavirus (HPV) capsid proteins L1 and L2 are expressed only in terminally differentiated cells.
  • This suggests HPV gene expression is regulated and potentially blocked in dividing cells.

Purpose of the Study:

  • To investigate the presence and function of cis-acting inhibitory sequences within the HPV type 16 L2 coding region.
  • To compare the regulatory elements in HPV-16 with those in HPV-1.

Main Methods:

  • Cloning the HPV-16 L2 coding region downstream of a reporter gene.
  • Analyzing mRNA and protein levels to assess inhibitory effects.
  • Performing deletion analysis to map inhibitory regions within L2.

Main Results:

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  • The HPV-16 L2 coding region contains orientation-dependent cis-acting inhibitory sequences.
  • Two inhibitory regions were identified: one in the 5' region (nucleotides 1-845) reducing mRNA stability, and a weaker one at the 3' end.
  • HPV type 1 L1 and L2 genes lacked similar strong inhibitory sequences.

Conclusions:

  • Inhibitory sequences in HPV-16 L1 and L2 mRNAs may regulate viral gene expression, potentially aiding immune evasion and persistent infections.
  • The absence of strong inhibitory sequences in HPV-1 correlates with higher viral production observed in HPV-1 infections.