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Increase of C-reactive protein and decrease of surfactant protein A in surfactant after lung transplantation
C Casals1, A Varela, M L Ruano
1Department of Biochemistry and Molecular Biology, Faculty of Biology, Complutense University, Madrid, Spain.
American Journal of Respiratory and Critical Care Medicine
|January 28, 1998
Summary
Serum C-reactive protein (CRP) increased in lung transplant surfactant, impairing its function. Elevated CRP in bronchoalveolar lavage may indicate lung injury after transplantation.
Area of Science:
- Pulmonary Medicine
- Transplantation Immunology
- Biochemistry
Background:
- Lung transplantation is a complex procedure with potential complications.
- Surfactant aggregates play a crucial role in maintaining lung function.
- Acute-phase proteins like C-reactive protein (CRP) are involved in inflammatory responses.
Purpose of the Study:
- To investigate the changes in surfactant aggregates after lung transplantation.
- To determine the effect of C-reactive protein (CRP) on surfactant function.
- To identify potential biomarkers for lung injury post-transplantation.
Main Methods:
- Single left lung transplantation was performed in dogs using two preservation solutions (EC and UW).
- Surfactant aggregates were isolated from transplanted lungs after 4.5 hours of reperfusion.
- Composition, interfacial adsorption activity, and CRP levels in surfactant were analyzed using Western blot and biochemical assays.
Main Results:
- Transplanted lungs showed impaired gas exchange and reduced surfactant adsorption capacity.
- Surfactant from transplanted lungs had decreased surfactant protein A and increased CRP levels.
- Addition of CRP to normal surfactant reduced its adsorption ability.
Conclusions:
- Impaired surfactant adsorption in transplanted lungs is linked to decreased surfactant protein A and increased CRP.
- Elevated CRP in bronchoalveolar lavage may serve as a sensitive marker for lung injury following transplantation.