Related Experiment Video
Updated: Aug 4, 2026
08:42
Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos
Published on: June 26, 2018
Sequence, structure, and chromosomal mapping of the mouse Lgals6 gene, encoding galectin-6
M A Gitt1, Y R Xia, R E Atchison
1Center for Neurobiology and Psychiatry, Department of Psychiatry, University of California, San Francisco, California 94143-0984, USA.
The Journal of Biological Chemistry
|February 28, 1998
Summary
Researchers isolated and characterized the mouse Lgals6 gene, encoding galectin-6, and a fragment of the Lgals4 gene, encoding galectin-4. These genes are located on mouse chromosome 7, near the apoE gene.
Area of Science:
- Genetics
- Molecular Biology
- Biochemistry
Background:
- Mouse gastrointestinal tract expresses two related galectins: galectin-4 and galectin-6.
- Both galectins possess two carbohydrate recognition domains within a single peptide.
Purpose of the Study:
- To isolate and characterize the complete mouse Lgals6 gene.
- To isolate and characterize a fragment of the mouse Lgals4 gene.
- To determine the chromosomal mapping of Lgals6 and Lgals4.
Main Methods:
- Gene isolation and characterization.
- Exon and promoter region analysis.
- Chromosomal mapping using genetic markers.
Main Results:
- The complete mouse Lgals6 gene, encoding galectin-6, was isolated and characterized.
- A fragment of the distinct Lgals4 gene, encoding galectin-4, was also isolated.
- The Lgals6 coding sequence is composed of eight exons.
- Two putative promoters were identified in the upstream region of Lgals6.
- Lgals6 and Lgals4 are clustered on mouse chromosome 7, approximately 3.2 centimorgans proximal to the apoE gene.
- The syntenic human chromosomal region is 19q13.1-13.3.
Conclusions:
- The genetic basis for galectin-4 and galectin-6 expression in the mouse gastrointestinal tract has been elucidated.
- The physical location and organization of the Lgals4 and Lgals6 genes provide insights into their coordinated regulation.
- Comparative mapping suggests conserved synteny between mouse chromosome 7 and human chromosome 19q13.1-13.3 for these galectin genes.
