Monitoring of blood catecholamines by microdialysis and microbore LC with a dual amperometric detector

The accuracy of in vivo microdialysis for monitoring blood catecholamines and their metabolites in Lan-Yu mini-pigs was evaluated. To prevent blood clots and irritation, a microdialysis probe was secured in a Y-shaped tube. The tube was connected to an arterio-venous shunt, in a mini-pig, for in vivo experiments. Perfusates were injected onto a microbore LC equipped with a dual electrochemical detector (the upstream electrode was set at an oxidizing potential and the downstream electrode was set at a reducing potential. The typical large offscale peak or interfering peaks on the anodic chromatograms were mostly eliminated on the cathodic chromatograms, thereby providing reliable measurements of early eluters. Early eluates, such as norepinephrine and epinephrine, with reversible redox behaviour could be detected at the downstream reducing electrode. A comparison of the present method and a conventional blood-drawing method showed good correlation (r = 0.775-0.983 for all analytes).