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High-performance liquid chromatographic determination of orotic acid as its methyl derivative in human urine
A Fioravanti1, M Flaviani, C Gambelunghe
1Department of Clinical Medicine, Pathology and Pharmacology, University of Perugia, Italy.
Journal of Chromatography. B, Biomedical Sciences and Applications
|February 3, 1998
Abstract:
Described is a method for the determination of orotic acid as its methyl ester in human urine. The method involves the use of solid-phase extraction to isolate pyrimidines from urine and derivatization with methanol and sulfuric acid, followed by isocratic high-performance liquid chromatography on a reversed-phase C18 column with UV absorbance detection. The assay is shown to be sufficiently sensitive for use in clinical investigations where elevated orotic acid excretion is suspected.