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Effect of phenobarbital on hepatic CYP1A1 and CYP1A2 in the Ahr-null mouse
H Zaher1, T J Yang, H V Gelboin
1Laboratory of Metabolism, National Cancer Institute, Bethesda, MD 20892, USA.
Biochemical Pharmacology
|February 4, 1998
Abstract:
Studies have suggested that phenobarbital (PB) induces members of the CYP1A subfamily by both transcriptional and post-transcriptional mechanisms. Using the Ahr -/- mice, we examined the induction of CYP1A1 and CYP1A2 by PB. CYP1A2 mRNA and protein were induced by PB in the null mice, suggesting that CYP1A2 is regulated by PB by a mechanism independent of the aryl hydrocarbon receptor (AHR). In contrast, the regulation of CYP1A1 is highly dependent on the AHR.