Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Auxin-induced developmental patterns in Brassica juncea embryos

K Hadfi1, V Speth, G Neuhaus

  • 1Institut fur Biologie II, Zellbiologie, Universitat Freiburg, Freiburg, Germany.

Development (Cambridge, England)
|May 9, 1998
PubMed
Summary

This study reveals how auxin regulates plant embryo development. Manipulating auxin levels and transport in Brassica juncea embryos caused various shape changes, mimicking developmental arrests and mutant phenotypes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Lobectomy of Right Lower Lobe Eight Years after Foreign Body Aspiration].

Pneumologie (Stuttgart, Germany)·2019
Same author

Individual selection, culture and manipulation of higher plant cells.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Intracellular localisation of phytochrome in oat coleoptiles by electron microscopy.

Planta·2013
Same author

Intracellular localisation of phytochrome and ubiquitin in red-light-irradiated oat coleoptiles by electron microscopy.

Planta·2013
Same author

Regeneration of fertile plants from excised immature zygotic embryos of Arabidopsis thaliana.

Plant cell reports·2013
Same author

Intracellular localisation of phytochrome in oat coleoptiles by electron microscopy : Dependence on light pretreatments and the amount of the active, far-red-absorbing form.

Planta·2013

Area of Science:

  • Plant biology
  • Developmental biology
  • Molecular genetics

Background:

  • Auxin is a key plant hormone regulating embryonic development.
  • Understanding auxin's role is crucial for deciphering pattern formation in dicot embryos.

Purpose of the Study:

  • To investigate the mechanism of auxin action during pattern formation in dicot embryos.
  • To analyze the effects of indole-3-acetic acid (IAA), N-(1-naphthyl)thalamic acid (NPA), and p-chlorophenoxyisobutyric acid (PCIB) on embryo development.

Main Methods:

  • In vitro treatments of isolated zygotic Brassica juncea embryos.
  • Application of exogenous auxin (IAA), auxin transport inhibitor (NPA), and antiauxin (PCIB).

Main Results:

  • Exogenous auxin inhibited morphogenesis or caused abnormal shapes (egg-, cucumber-shaped).

Related Experiment Videos

  • Auxin transport inhibition led to axis duplication (twin embryos) or axis broadening (two radicles).
  • Antiauxin treatment affected cotyledon and hypocotyl/radicle development, resulting in single/no cotyledons or inhibited growth.

    • Conclusions:

    • Observed morphogenetic alterations represent arrests in auxin-regulated developmental steps.
    • These experimentally induced changes phenocopy known Arabidopsis morphogenetic mutants.
    • This study provides insights into auxin's precise role in establishing embryonic patterns.