Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Preconditioning human cardiomyocytes and endothelial cells

T Shirai1, V Rao, R D Weisel

  • 1Department of Clinical Biochemistry, Toronto Hospital, Ontario, Canada.

The Journal of Thoracic and Cardiovascular Surgery
|February 6, 1998
PubMed
Summary

Simulated ischemia and reperfusion caused more injury to endothelial cells than cardiomyocytes. Preconditioning protected cardiomyocytes but not endothelial cells from ischemia-reperfusion injury.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of a respiratory virus panel test for detection of twenty human respiratory viruses by use of multiplex PCR and a fluid microbead-based assay.

Journal of clinical microbiology·2007
Same author

Peri-operative supplemental warming was associated with fewer morbid cardiac events than routine thermal care.

Evidence-based cardiovascular medicine·2005
Same author

CD3 monitoring and thymoglobulin therapy in cardiac transplantation: clinical outcomes and pharmacoeconomic implications.

Transplantation proceedings·2005
Same author

Angiogenesis: protein, gene, or cell therapy?

The heart surgery forum·2002
Same author

Optimal time for cardiomyocyte transplantation to maximize myocardial function after left ventricular injury.

The Annals of thoracic surgery·2002
Same author

Angiogenesis by endothelial cell transplantation.

The Journal of thoracic and cardiovascular surgery·2001

Area of Science:

  • Cardiovascular Biology
  • Cellular Physiology
  • Ischemia-Reperfusion Injury

Background:

  • Investigates the impact of simulated ischemia and reperfusion on human ventricular cardiomyocytes and endothelial cells.
  • Examines cellular responses to varying oxygen tensions during ischemic events.

Purpose of the Study:

  • To evaluate the differential susceptibility of cardiomyocytes and endothelial cells to ischemia-reperfusion injury.
  • To assess the protective effects of preconditioning on these cell types.

Main Methods:

  • Cell cultures exposed to hypoxic or anoxic conditions simulating ischemia, followed by reperfusion.
  • Cell injury assessed via trypan blue exclusion; lactate and acid release measured.

Main Results:

Related Experiment Videos

  • Endothelial cells exhibited greater injury than cardiomyocytes under both hypoxic and anoxic ischemia.
  • Anoxic ischemia induced more severe injury than hypoxic ischemia in both cell types.
  • Preconditioning reduced injury and lactate accumulation in cardiomyocytes but not endothelial cells.

Conclusions:

  • Endothelial cells are more vulnerable to ischemia-reperfusion injury compared to cardiomyocytes.
  • Preconditioning confers protection to cardiomyocytes but not endothelial cells against ischemia-reperfusion insult.