Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Flow cytometry in diagnostic cytology

T J O'Leary1

  • 1Department of Cellular Pathology, Armed Forces Institute of Pathology, Washington, DC 20306-6000, USA.

Diagnostic Cytopathology
|February 6, 1998
PubMed
Summary

Flow cytometry (FCM) enhances cytologic examination by providing quantitative data alongside morphology. However, advancements in image cytometry and AI pose challenges to FCM

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preface to the special issue of <i>BMJ Military Health</i> on future soldier: delivering human advantage.

BMJ military health·2025
Same author

Self-reported hormonal contraceptive use in the British Armed Forces.

BMJ military health·2025
Same author

MSKI reduction strategies: evidence-based interventions to reduce musculoskeletal injuries in military service members.

BMJ military health·2024
Same author

Protein dose requirements to maximize skeletal muscle protein synthesis after repeated bouts of resistance exercise in young trained women.

Scandinavian journal of medicine & science in sports·2023
Same author

Effect of sex and combat employment on musculoskeletal injuries and medical downgrading in trained military personnel: an observational cohort study.

BMJ military health·2023
Same author

Hormonal contraceptive use, bone density and biochemical markers of bone metabolism in British Army recruits.

BMJ military health·2021

Area of Science:

  • Cytopathology
  • Biochemistry
  • Immunology

Background:

  • Flow cytometry (FCM) is a valuable tool in cytopathology, complementing morphologic analysis with quantitative biochemical data.
  • It is particularly useful for analyzing bladder washings and lymphoid lesions from various sample types.
  • Optimal FCM analysis often involves multiparameter assessments, such as combining cell differentiation markers with DNA quantitation.

Purpose of the Study:

  • To evaluate the current utility and future prospects of flow cytometry in cytopathology.
  • To compare flow cytometry with emerging technologies like image cytometry and immunocytochemistry.
  • To identify areas for technical refinement to maintain flow cytometry's role in cytopathology.

Main Methods:

  • Review of flow cytometry applications in cytopathology, including its use in analyzing bladder washings and lymphoid lesions.
  • Comparison of flow cytometry's capabilities with those of image cytometry, artificial intelligence (AI) methods, and immunocytochemistry.
  • Assessment of multiparameter analysis in flow cytometry versus imaging techniques.

Main Results:

  • Flow cytometry provides crucial quantitative biochemical information that complements morphologic examination.
  • Image cytometry offers advantages in assessing ploidy, S-fraction, and multiparameter measurements, with visual identification of cells.
  • AI development in imaging has surpassed that of FCM, and immunocytochemistry competes in proliferation assessment.

Conclusions:

  • Flow cytometry is a useful ancillary technique in cytopathology, especially for lymphoid lesions and bladder washings.
  • Image cytometry and AI present significant competition due to their advanced capabilities and cost-effectiveness.
  • Further technical refinements are necessary for flow cytometry to remain competitive against image cytometry and immunocytochemistry.

Related Experiment Videos