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Updated: Aug 25, 2026

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis
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T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis

Published on: July 31, 2010

Haptoglobin--haemoglobin interaction. Heterogeneity of the haptoglobin 1-1 molecule in its binding affinity for horse

F Lavialle, M Rogard, A Alfsen

    European Journal of Biochemistry
    |April 15, 1976
    PubMed

    The formation of two different complexes when haptoglobin (Hp) and haemoglobin (Hb) are mixed in a 1:1 molar ratio is demonstrated by isoelectrofocusing. In these two complexes, the affinity of Hp for Hb is shown to be different, since Hb can be displaced only from one of the complexes, by a further addition of Hp. This is confirmed by a quantitative study of the reaction stoichiometry, when [Hp]/[Hb] = 1 and [Hp]/[Hb] greater than 1, which allows an evaluation of the amount of each complex formed. All these data cannot be explained other than by the existence of two forms of Hp molecule and a reaction scheme which fits these experiments is proposed.

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