Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mucin gene expression in biliary epithelial cells

B Vandenhaute1, M P Buisine, V Debailleul

  • 1Laboratoire d'Anatomie et Cytologie Pathologique de l'Hôpital C. Huriez, CHRU de Lille, Unité INSERM 377, France.

Journal of Hepatology
|February 7, 1998
PubMed
Summary

Human mucin gene expression in the biliary tree was investigated. MUC3, MUC6, and MUC5B showed strong mRNA expression in biliary epithelial cells, with MUC3 also found in hepatocytes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Search for Neutron-to-Hidden-Neutron Oscillations in an Ultracold Neutron Beam.

Physical review letters·2023
Same author

3D multi-cell-type liver organoids: A new model of non-alcoholic fatty liver disease for drug safety assessments.

Toxicology in vitro : an international journal published in association with BIBRA·2023
Same author

[Central toxic keratopathy: A complication of femtoLASIK refractive surgery].

Journal francais d'ophtalmologie·2023
Same author

Publisher's Note: "The very large n2EDM magnetically shielded room with an exceptional performance for fundamental physics measurements" [Rev. Sci. Instrum. 93, 095105 (2022)].

The Review of scientific instruments·2022
Same author

The very large n2EDM magnetically shielded room with an exceptional performance for fundamental physics measurements.

The Review of scientific instruments·2022
Same author

The design of the n2EDM experiment: nEDM Collaboration.

The European physical journal. C, Particles and fields·2021

Area of Science:

  • Gastroenterology
  • Molecular Biology
  • Cell Biology

Background:

  • Human mucin gene knowledge has advanced, but expression in the biliary tree remains largely unknown.
  • This study focuses on mucin gene expression within the gallbladder, intrahepatic bile ducts, and liver.

Purpose of the Study:

  • To determine the expression patterns of various human mucin genes in the biliary tree.
  • To identify key mucin genes expressed in gallbladder, intrahepatic bile ducts, and liver cells.

Main Methods:

  • Utilized Northern-blot and in situ hybridization techniques.
  • Examined expression using probes for eight mucin genes: MUC1, MUC2, MUC3, MUC4, MUC5AC, MUC5B, MUC6, and MUC7.

Main Results:

  • Strong mRNA expression observed for MUC3, MUC6, and MUC5B.

Related Experiment Videos

  • Weak expression detected for MUC1, MUC5AC, and MUC2; no expression found for MUC4 and MUC7.
  • MUC3, the most expressed gene in the biliary tree, was also identified in hepatocytes, suggesting a non-secreted function.

    • Conclusions:

    • MUC3, MUC6, and MUC5B are the primary mucin genes expressed in biliary epithelial cells.
    • The presence of MUC3 in hepatocytes indicates a potential novel role beyond mucin secretion.