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Multiple false-positive reactions in anti-CD4 ELISA testing of dystrophic children
Background:
Anti-CD4 autoantibodies are present in up to 10% of HIV infected patients and, until present, they were not recorded in other pathological circumstances.
Aim:
To test the presence of anti-CD4 autoantibodies in HIV-infected children, using an indirect ELISA test.
Design:
Cross-sectional controlled study.
Patients And Methods:
The study group: 9 HIV infected dystrophic children--3 AIDS (P2A), 6 P1C. Control groups: 9 HIV seronegative dystrophic children; 14 HIV seronegative eutrophic patients presenting chronic hepatopathies, or dialysed patients.
Methods:
1. Indirect ELISA using as antigen sCD4 molecules; 2. Western blot (sCD4); 3. "Antigenic displacement": sera preincubation with sCD4, followed by indirect ELISA.
Results:
Indirect ELISA revealed anti-CD4 reactivities in all the dystrophic patients (9 HIV+ and 9 HIV-). WB sCD4 did not confirmed none of these reactivities. Sera preincubation with sCD4 did not significantly modified ELISA CD4 reactivities.
Conclusions:
False positive results (FPR) rate following testing of the presence of anti-CD4 in dystrophic children recommends prudence for the interpretation of such tests. "Antigenic displacement" could be an accessible and easy to perform method in order to eliminate the FPR.
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