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Developments in amino acid analysis using capillary electrophoresis

J T Smith1

  • 1Department of Physical Sciences, Southeastern Oklahoma State University, Durant 74701-0609, USA. tsmith@sosu.edu

Electrophoresis
|February 10, 1998
PubMed
Summary
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Capillary electrophoresis (CE) is a powerful technique for amino acid analysis. This review covers derivatization, separation, and detection methods, highlighting advances in enantiomeric resolution and applications.

Area of Science:

  • Biochemistry
  • Analytical Chemistry

Background:

  • Amino acid analysis is crucial in biological sciences.
  • Capillary electrophoresis (CE) has emerged as a key technique for this analysis.
  • Over 200 articles published in the last five years indicate growing interest in CE for amino acid analysis.

Purpose of the Study:

  • To review the current status of amino acid analysis using capillary electrophoresis.
  • To discuss advancements in derivatization, separation, and detection techniques.
  • To summarize progress in enantiomeric resolution methodologies for amino acids.

Main Methods:

  • Review of literature published between 1991 and 1996.
  • Discussion of pre-, post-, and on-column derivatization techniques.
  • Presentation of various separation and enantiomeric resolution methodologies.

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  • Overview of recent advances in detection strategies.

    • Main Results:

    • Several separation methodologies enabling resolution of large amino acid sets are presented.
    • Advances in both direct and indirect enantiomeric separation schemes are summarized.
    • Recent developments in detection strategies for derivatized and underivatized amino acids are detailed.

    Conclusions:

    • Capillary electrophoresis offers versatile and advanced solutions for comprehensive amino acid analysis.
    • The field has seen significant progress in derivatization, separation, and detection techniques.
    • CE is a valuable tool for diverse applications in biological analysis.