Counting method of live fat cells used in lipoinjection procedures

Area of Science:

• Regenerative Medicine
• Adipose Tissue Engineering

Background:

• Lipoinjection procedures rely on the viability of transferred adipose tissue.
• Quantifying live fat cells is crucial for predicting successful fat grafting outcomes.
• Current methods lack precise quantification of viable adipocytes in autologous fat grafts.

Purpose of the Study:

• To establish a technical procedure for quantifying live fat cells in lipoinjection samples.
• To assess the impact of blood contamination on live fat cell viability.
• To determine the influence of injection tools on fat cell survival.

Main Methods:

• Development of a novel quantification technique for live adipocytes.
• Analysis of aspirated fat samples intended for lipoinjection.
• Correlation analysis between blood content and live fat cell count.
• Comparison of fat cell viability using different injection apparatus (syringe vs. cannula with pump).

Main Results:

• A reliable method to quantify live fat cells per cubic millimeter of injection volume was established.
• A statistically significant inverse correlation exists between blood volume and live fat cell count.
• Injection method (syringe or cannula with pump) showed no significant impact on fat cell viability in 95% of cases.

Conclusions:

• The developed method allows for precise enumeration of viable fat cells crucial for lipoinjection.
• Minimizing blood contamination in fat grafts is essential to maximize live fat cell yield and improve graft survival.
• The choice of injection tool appears to be less critical than sample quality for fat cell viability.