Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapid detection of D-dimer using a fiber optic biosensor

C A Rowe1, J S Bolitho, A Jane

  • 1Naval Research Laboratory, Center for Bio/Molecular Science and Engineering, Washington, DC 20375-5438, USA.

Thrombosis and Haemostasis
|February 12, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

UNDERSTANDING THE LONG-TERM INTERPLAY BETWEEN GLUCOCORTICOIDS, PARATHYROID HORMONE LEVELS, AND OSTEOPOROSIS IN PATIENTS.

Georgian medical news·2023
Same author

LIVER FIBROSIS: PATHOPHYSIOLOGY, DIAGNOSIS, AND EMERGING THERAPEUTIC TARGETS FOR A COMMON COMPLICATION OF CHRONIC LIVER DISEASES.

Georgian medical news·2023
Same author

Presence of receptors for self MHC antigens on non-T cells.

Immunology today·2014
Same author

Patterned planar array immunosensor for multianalyte detection.

Journal of biomedical optics·2012
Same author

Automated processing integrated with a microflow cytometer for pathogen detection in clinical matrices.

Biosensors & bioelectronics·2012
Same author

A computational reaction-diffusion model for the analysis of transport-limited kinetics.

Analytical chemistry·2011
Same journal

Discontinuation of Oral Anticoagulation After Successful Catheter Ablation for Atrial Fibrillation: Meta-analysis of Randomized Clinical Trials.

Thrombosis and haemostasis·2026
Same journal

Novel/Recurrent Variants in Pakistani Glanzmann Thrombasthenia and Glanzmann-like bleeding diathesis: Insights from NGS Analysis.

Thrombosis and haemostasis·2026
Same journal

Anti-TFPI Single-Domain Antibodies: Novel Rebalancing Therapies for Hemophilia and Other Rare Bleeding Disorders.

Thrombosis and haemostasis·2026
Same journal

The Interaction of Fibrin with Endothelial Cell Receptor N-Cadherin Promotes Fibrin-Dependent Angiogenesis.

Thrombosis and haemostasis·2026
Same journal

Antiphospholipid Antibodies and Atrial Fibrillation: Clinical Implications and the TaPL AF Study Design.

Thrombosis and haemostasis·2026
Same journal

Prasugrel vs. Ticagrelor: Can TUXEDO-2 Settle the Debate?

Thrombosis and haemostasis·2026
See all related articles

A new fiber optic biosensor rapidly detects crosslinked fibrin degradation products (XL-FDPs), including D-dimer, in under 12 minutes. This accurate diagnostic tool shows promise for sepsis and thrombotic disorder patient monitoring.

Area of Science:

  • Biomedical Engineering
  • Clinical Chemistry
  • Biotechnology

Background:

  • Elevated crosslinked fibrin degradation products (XL-FDPs), including D-dimer, are biomarkers for sepsis and thrombotic disorders.
  • Accurate and rapid quantification of these markers is crucial for timely diagnosis and patient management.

Purpose of the Study:

  • To develop and validate a rapid, sensitive fiber optic biosensor for detecting and quantifying D-dimer and other XL-FDPs.
  • To assess the biosensor's performance in buffer and plasma samples, including those from septic patients.

Main Methods:

  • Utilized a sandwich fluoroimmunoassay immobilized on an evanescent wave biosensor.
  • Employed calibrated fiber optics for signal detection.
  • Validated results against Enzyme-Linked Immunosorbent Assay (ELISA) using samples from septic patients.

Related Experiment Videos

Main Results:

  • Achieved detection and quantification of D-dimer and high molecular weight XL-FDPs in 11 minutes.
  • Demonstrated strong correlation (r = 0.918) between fiber optic assay and ELISA results.
  • Reported intra- and inter-assay errors comparable to ELISA.

Conclusions:

  • The developed fiber optic biosensor offers accurate and rapid detection of XL-FDPs.
  • This technology shows significant potential as a diagnostic tool for sepsis and thrombotic disorders.
  • The method's speed and sensitivity facilitate quicker clinical decision-making.