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Source of extracellular brain adenosine during hypoxia in fetal sheep
B J Koos1, L Kruger, T F Murray
1Department of Obstetrics and Gynecology, Brain Research Institute, UCLA School of Medicine, Los Angeles, CA 90095-1740, USA.
Abstract:
Microdialysis was performed to determine whether hypoxia increases fetal brain adenosine (ADO) concentration through dephosphorylation of extracellular 5'-adenosine monophosphate (5-AMP). Hypoxia (fetal PaO2 approximately 14 Torr) increased fetal brain ADO levels approximately two-fold when the probes were perfused with synthetic cerebrospinal fluid (CSF) containing inhibitors of the nucleoside transporter but not with this solution plus a blocker of ecto-5'-nucleotidase (AOPCP). The hypoxia-induced rise in fetal brain ADO concentrations depends critically upon the hydrolysis of extracellular 5'-AMP.