Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Adjuvant lipopeptide interaction with model membranes

J Gonzalez-Christen1, I Vergne, R Süssmuth

  • 1Institut de Pharmacologie et de Biologie Structurale du CNRS and Université Paul Sabatier, Toulouse, France.

Biochimica Et Biophysica Acta
|February 12, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antibacterial Activity of combinatorial treatments composed of transition-metal/antibiotics against Mycobacterium tuberculosis.

Scientific reports·2019
Same author

[Is there a relationship between cystatin C and inflammatory status, oxidative stress and other cardiovascular risk factors in non-diabetic patients with chronic kidney disease?].

Nefrologia : publicacion oficial de la Sociedad Espanola Nefrologia·2009
Same author

Surface active molecules: preparation and properties of long chain n-acyl-l-alpha-amino-omega-guanidine alkyl acid derivatives.

International journal of cosmetic science·2009
Same author

Genome analysis of Bacillus amyloliquefaciens FZB42 reveals its potential for biocontrol of plant pathogens.

Journal of biotechnology·2008
Same author

[68 year-old male with aneurysm of ascending aorta].

Revista clinica espanola·2007
Same author

Bioassay guided isolation of a new C18-polyacetylene, (+)-9(Z),17-octadecadiene-12,14-diyne-1,11,16-triol, from Cassonia barteri.

Planta medica·2007
Same journal

Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Molecular Basis of Disease Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

General Subjects Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

Biochimica et biophysica acta·2019
Same journal

Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

Biochimica et biophysica acta·2019
Same journal

Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

Biochimica et biophysica acta·2019
See all related articles

The cationic lipohexapeptide Pam3Cys-Ser-(Lys)4 interacts with lipid interfaces, influencing vesicle aggregation and inhibiting calcium-induced fusion. This lipopeptide shows potential in modulating membrane properties and calcium binding.

Area of Science:

  • Biochemistry
  • Surface Chemistry
  • Membrane Biophysics

Background:

  • The cationic lipohexapeptide Pam3Cys-Ser-(Lys)4 mimics bacterial lipoproteins, acting as an adjuvant and macrophage activator.
  • Understanding its interaction with lipid membranes is crucial for its application in drug delivery and immunology.

Purpose of the Study:

  • To investigate the interfacial behavior and membrane interaction of the lipohexapeptide Pam3Cys-Ser-(Lys)4.
  • To elucidate the effects of this lipopeptide on lipid vesicle aggregation, fusion, and calcium binding.

Main Methods:

  • Surface potential and surface pressure measurements at the air-water interface with a phosphatidylserine monolayer.
  • Interaction studies with large unilamellar vesicles (LUVs) and small unilamellar vesicles (SUVs).

Related Experiment Videos

  • Monitoring of LUV aggregation, lipid exchange, and calcium-induced fusion inhibition.
  • 45Ca2+ displacement assays from phosphatidylserine monolayers.

    • Main Results:

    • The lipopeptide interacted with and inserted into phosphatidylserine monolayers, reducing surface potential and increasing surface pressure until charge neutralization.
    • Insertion was limited at high surface pressures (above 29 mN/m).
    • Kinetics of interaction depended on ionic strength and lipid acyl chain properties, but equilibrium was independent.
    • Lipopeptide induced LUV aggregation and lipid exchange, but not fusion.
    • It strongly inhibited calcium-induced fusion of PS LUVs by displacing Ca2+ from the monolayer.
    • Phospholipid segregation was observed in SUVs at micromolar concentrations.

    Conclusions:

    • Pam3Cys-Ser-(Lys)4 exhibits complex interfacial behavior and modulates membrane properties.
    • The lipopeptide's ability to inhibit calcium-induced fusion suggests a role in controlling membrane dynamics and calcium signaling.
    • These findings provide insights into the mechanism of action for this synthetic lipoprotein model.