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Inheritance of CYP6D1-mediated pyrethroid resistance in house fly (Diptera: Muscidae)
Abstract:
The inheritance of cytochrome P450 monooxygenase-mediated (i.e., CYP6D1) resistance in the LPR strain of house fly, Musca domestica L., was investigated by comparing levels of monooxygenase-mediated permethrin resistance and CYP6D1 messenger RNA in 7 populations of house flies. Results indicate that monooxygenase-mediated resistance, and overexpression of CYP6D1 in the LPR strain are controlled by an incompletely recessive trans acting factor(s) on autosome 2 and a nearly completely dominant cis acting factor(s) on autosome 1. The polygenic nature of CYP6D1-mediated resistance and the relative dominance of the resistance factor(s) on autosome 1 suggests this type of resistance could develop relatively quickly.
Insights
Inheritance of insecticide resistance in house flies (Musca domestica L.) is linked to cytochrome P450 monooxygenase (CYP6D1). Genetic factors on autosomes 1 and 2 control this resistance, influencing permethrin tolerance.
Area of Science:
- Entomology
- Genetics
- Biochemistry
Background:
- House flies (Musca domestica L.) exhibit resistance to insecticides like permethrin.
- Cytochrome P450 monooxygenases, specifically CYP6D1, are implicated in mediating this resistance.
- Understanding the genetic basis of insecticide resistance is crucial for pest management.
Purpose of the Study:
- To investigate the inheritance patterns of CYP6D1-mediated permethrin resistance in the LPR strain of house flies.
- To identify the genetic factors controlling CYP6D1 overexpression and associated resistance.
- To determine the mode of inheritance and dominance of these genetic factors.
Main Methods:
- Comparing permethrin resistance levels across seven house fly populations.
- Quantifying CYP6D1 messenger RNA (mRNA) levels in these populations.
- Analyzing the genetic control of resistance through inheritance studies.
Main Results:
- Monooxygenase-mediated resistance and CYP6D1 overexpression in the LPR strain are controlled by genetic factors.
- An incompletely recessive trans-acting factor on autosome 2 influences resistance.
- A nearly completely dominant cis-acting factor on autosome 1 also plays a significant role.
Conclusions:
- CYP6D1-mediated insecticide resistance in house flies is polygenic.
- The dominance of resistance factors on autosome 1 suggests rapid evolution of resistance.
- This genetic understanding can inform strategies to manage insecticide resistance in pest populations.