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Activity of new quinolones against intracellular Mycobacterium avium in human monocytes
N Venkataprasad1, M R Jacobs, J L Johnson
1Division of Infectious Diseases, Case Western Reserve University, and University Hospitals of Cleveland, OH 44106, USA. nvenkata@rpms.ac.uk
Abstract:
The ability to inhibit the in-vitro growth of mycobacteria within human monocytes is a useful screening assay for novel chemotherapeutic agents. In this study the MICs of a panel of new quinolones were determined by the broth microdilution method for two strains of Mycobacterium avium. Sixteen such compounds with MIC90s ranging from 2 to >32 mg/L were subsequently selected for the 7 day monocyte assay using ciprofloxacin for comparison. The degree of inhibition of intracellular growth correlated with the MICs. PD 139586, PD 143289, PD 135144, PD 119421 and PD 131575 were the most active new agents with activities superior to those of ciprofloxacin and sparfloxacin.