Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Effect of sample volume on quantitative analysis by solid-phase microextraction. Part 1. Theoretical considerations

T Górecki1, J Pawliszyn

  • 1Department of Chemistry and Waterloo Centre for Groundwater Research, University of Waterloo, Ontario, Canada.

The Analyst
|February 17, 1998
PubMed
Summary

Sample volume significantly impacts solid-phase microextraction (SPME) results. Researchers calculated minimum sample volumes for accurate analyte extraction and determined optimal conditions for partition coefficient (K) determination in SPME analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Experimentally validated mathematical model of analyte uptake by permeation passive samplers.

Environmental science. Processes & impacts·2017
Same author

Identification of oxidative coupling products of xylenols arising from laboratory-scale phytoremediation.

Chemosphere·2014
Same author

Pharmacokinetics of tranexamic acid in patients undergoing cardiac surgery with use of cardiopulmonary bypass.

Anaesthesia·2012
Same author

New solvent-free sample preparation techniques.

Environmental science & technology·2012
Same author

Analysis of environmental air samples by solid-phase microextraction and gas chromatography/ion trap mass spectrometry.

Environmental science & technology·2011
Same author

Rapid determination of polyaromatic hydrocarbons and polychlorinated biphenyls in water using solid-phase microextraction and GC/MS.

Environmental science & technology·2011

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Solid-phase microextraction (SPME) is a widely used technique for analyte extraction.
  • The influence of sample volume on SPME efficiency and accuracy is a critical consideration.

Purpose of the Study:

  • To investigate the effect of sample volume on analyte extraction in two- and three-phase SPME systems.
  • To introduce new concepts and summarize current knowledge regarding sample volume effects.
  • To provide calculated values for minimum sample volumes and volumes for 50% analyte extraction.

Main Methods:

  • Theoretical calculations for two-phase (sample-fiber coating) and three-phase (sample-headspace-fiber coating) SPME systems.
  • Analysis of extraction kinetics and headspace capacity effects.
  • Discussion of partition coefficient (K) determination accuracy.

Related Experiment Videos

Main Results:

  • Sample volume significantly affects quantification and precision in SPME, except in rare cases.
  • Minimum sample volumes were calculated to ensure analyte extraction is <1% of the initial amount.
  • Volumes for 50% analyte extraction were determined for both two- and three-phase systems.
  • Equilibration in headspace SPME is fast with large headspace capacity but reduces sensitivity.
  • Accurate K value determination requires accounting for analyte concentration changes and using large sample volumes.

Conclusions:

  • Maintaining consistent sample and standard volumes is crucial for SPME analysis.
  • Large sample volumes are recommended for accurate partition coefficient (K) determination.
  • Understanding and controlling sample volume effects are essential for reliable SPME results.