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Complete nucleotide sequence of human NRAMP2 cDNA

F Kishi1, M Tabuchi

  • 1Center for Gene Research, Yamaguchi University, Ube, Japan.

Molecular Immunology
|August 1, 1997
PubMed

Insights

Researchers isolated and characterized human NRAMP2, a gene related to macrophage antimicrobial activity. Its distinct N-terminal domain suggests unique functions compared to NRAMP1, impacting pathogen defense.

Area of Science:

  • Molecular Biology
  • Immunology
  • Genetics

Background:

  • The Lsh/Ity/Bcg locus in mice influences macrophage activation against intracellular pathogens.
  • The Nramp1 gene was identified as a key candidate within this locus.

Purpose of the Study:

  • To isolate and characterize the full-length cDNA for human NRAMP2.
  • To compare the sequence and potential function of NRAMP2 with NRAMP1.

Main Methods:

  • Isolation and sequencing of full-length human NRAMP2 cDNA.
  • Bioinformatic analysis of predicted amino acid sequences.
  • Comparative sequence analysis between NRAMP1 and NRAMP2.

Main Results:

  • A 4142 bp cDNA for human NRAMP2 was isolated, coding for a 561-amino acid protein (61,456 Da).
  • NRAMP1 and NRAMP2 share 64% overall sequence identity.
  • Only 21% identity was observed in the N-terminal cytoplasmic domain, where NRAMP1 interacts with microtubules.

Conclusions:

  • Human NRAMP2 has been successfully isolated and characterized.
  • Significant sequence divergence in the N-terminal domain suggests NRAMP2 may possess distinct functions compared to NRAMP1, potentially in cellular processes beyond antimicrobial activity.

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