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Related Experiment Videos

High-resolution YAC-cosmid-STS map of human chromosome 13

E Cayanis1, J J Russo, S Kalachikov

  • 1Columbia Genome Center, Columbia University, New York, New York 10032, USA.

Genomics
|February 18, 1998
PubMed
Summary

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Researchers created a detailed physical map of human chromosome 13, covering 98 Mb of the 13q region with yeast artificial chromosomes (YACs) and cosmids. This map includes 655 sequence-tagged sites (STSs) to aid gene discovery and disease research.

Area of Science:

  • Genomics
  • Human Genetics
  • Molecular Biology

Background:

  • High-resolution physical maps are essential for understanding genome organization and function.
  • Chromosome 13 plays a role in various hereditary diseases, necessitating detailed mapping.

Purpose of the Study:

  • To assemble a high-resolution physical map of human chromosome 13.
  • To facilitate the localization of additional markers, provide sequencing substrates, and aid in the discovery of chromosome 13 genes.

Main Methods:

  • Utilized hybridization, Polymerase Chain Reaction (PCR), and Fluorescence In Situ Hybridization (FISH) mapping data.
  • Employed a specifically designed set of computer programs for map assembly.
  • Integrated yeast artificial chromosomes (YACs) and cosmids to create a contig map of chromosome 13q.

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Main Results:

  • Assembled a physical map of human chromosome 13 (114 Mb), with 13q (98 Mb) covered by a near-continuous contig of 736 YACs and 597 cosmid contigs.
  • Annotated the 13q map with 655 sequence-tagged sites (STSs), averaging 1 STS per 150 kb.
  • Included database markers, expressed sequence tags, and cosmid end sequences within the STS set, alongside 197 FISH-mapped cosmids.

Conclusions:

  • The comprehensive physical map of human chromosome 13 is a valuable resource for genetic research.
  • Facilitates accurate marker localization, provides substrates for sequencing, and aids in identifying genes linked to hereditary diseases.
  • The map and associated data are publicly available online for scientific use.