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[The receptor and pathways for human cytomegalovirus entry]
1Department of Virology, University of Tokyo.
Nihon Rinsho. Japanese Journal of Clinical Medicine
|February 18, 1998
Summary
Human cytomegalovirus (HCMV) entry into neuroectodermal cells occurs independently of CD13. This study reveals a novel HCMV entry pathway in neuroblastoma cells, distinct from fibroblast cell interactions.
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Background:
- The cellular receptor mediating human cytomegalovirus (HCMV) entry remains unidentified.
- Heparan sulfate proteoglycan, annexin II, and CD13 (aminopeptidase N) have been implicated in HCMV entry into human fibroblasts.
Purpose of the Study:
- To investigate the role of heparan sulfate and CD13 in HCMV entry into neuroectodermal-derived cell lines.
- To elucidate the HCMV entry pathway in neuroectodermal tissues.
Main Methods:
- Generation of a recombinant HCMV expressing a marker protein.
- Infection assays using human neuroblastoma and malignant melanoma cell lines with varying expression of heparan sulfate and CD13.
Main Results:
- Recombinant HCMV successfully infected neuroblastoma cell lines lacking CD13 but expressing heparan sulfate.
- HCMV entry was inhibited in a malignant melanoma cell line expressing CD13.
- Evidence suggests a CD13-independent HCMV entry mechanism into neuroectodermal cells.
Conclusions:
- HCMV entry into neuroectodermal tissue-derived cells does not rely on CD13.
- The findings suggest a distinct HCMV cellular entry pathway in neuroectodermal cells compared to fibroblasts.