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Related Experiment Videos

Transgenic mouse models for tumor suppressor genes

G S Palapattu1, S Bao, T R Kumar

  • 1Department of Pathology, Baylor College of Medicine, Houston, TX 77030, USA.

Cancer Detection and Prevention
|February 18, 1998
PubMed
Summary

Mice lacking tumor suppressor genes are susceptible to tumors, proving mice are a potent assay for gene function. The alpha-inhibin gene

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Area of Science:

  • Cancer biology
  • Genetics
  • Molecular biology

Background:

  • Tumor suppressor genes are typically identified through human cancer syndromes and reverse genetics.
  • Gene targeting in mouse embryonic stem (ES) cells enables the creation of mutant mouse models that mimic human genetic disorders.

Purpose of the Study:

  • To evaluate the in vivo function of tumor suppressor genes.
  • To establish the mouse as an in vivo assay system for tumor suppressor genes.
  • To explore the utility of this system for identifying novel tumor suppressor genes.

Main Methods:

  • Generation of mutant mouse models using gene targeting in ES cells.
  • Analysis of tumor development in mice lacking specific tumor suppressor gene alleles.
  • Focus on gonadal tumor development in mice lacking alpha-inhibin gene copies.

Main Results:

  • Mice lacking tumor suppressor gene alleles exhibit high susceptibility to tumor development.
  • The absence of both alpha-inhibin gene copies in mice initiates gonadal tumor development.
  • This demonstrates the mouse's efficacy as an in vivo assay for tumor suppressor genes.

Conclusions:

  • The mouse serves as a powerful in vivo system for studying tumor suppressor genes.
  • The alpha-inhibin gene's role in tumor suppression is confirmed through mouse models.
  • Future research will leverage murine ES cell/gene targeting for novel tumor suppressor discovery and functional analysis.

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