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Bacteriophage T7 mRNA is polyadenylated

M D Johnson1, J Popowski, G J Cao

  • 1Boston Biomedical Research Institute, MA 02114, USA.

Molecular Microbiology
|February 18, 1998
PubMed
Summary

Bacterial virus RNA, specifically bacteriophage T7 mRNA, is polyadenylated, similar to bacterial messenger RNA (mRNA). This polyadenylation occurs after RNA processing and sometimes even during degradation.

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Area of Science:

  • Molecular Biology
  • Virology
  • Genetics

Background:

  • Bacterial messenger RNA (mRNA) is known to possess polyadenylated tails.
  • The polyadenylation status of viral RNA in bacteria, particularly bacteriophages, has not been extensively studied.

Purpose of the Study:

  • To investigate whether bacteriophage T7 RNA transcripts are polyadenylated.
  • To characterize the location and timing of polyadenylation in bacteriophage T7 mRNA.

Main Methods:

  • Analysis of pulse-labeled and steady-state bacteriophage T7 RNA using oligo(dT) cellulose binding and gene-specific probe hybridization.
  • Screening of oligo(dT)-dependent complementary DNA (cDNA) synthesis products.
  • Preparation and sequencing of a cDNA library from bacteriophage T7-infected cells to identify polyadenylation sites.

Main Results:

  • Polyadenylation was detected in early, middle, and late bacteriophage T7-specific mRNA transcripts.
  • Sequencing revealed polyadenylation sites located near the end of protein-coding regions, including one at an RNase III processing site.
  • Some polyadenylation events were observed within protein-coding regions.

Conclusions:

  • Bacteriophage T7 mRNA exhibits polyadenylation, a feature previously thought to be exclusive to bacterial mRNA.
  • Polyadenylation appears to occur post-transcriptionally, following nucleolytic processing of primary transcripts.
  • Polyadenylation may also occur concurrently with or after the initiation of mRNA degradation.

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