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Related Experiment Video

Updated: Jul 20, 2026

Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis

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A simple and efficient urine sampling method for bacteriological examination in elderly women

W J Michielsen¹, F J Geurs, G L Verschraegen

¹Department of Geriatrics and Gerontology, University Hospital, Gent, Belgium.

|February 18, 1998

Summary

A simple method of collecting voided urine samples accurately diagnoses urinary tract infections in elderly women. This technique can replace more invasive methods like suprapubic aspiration and catheterization, improving patient comfort and reducing healthcare burdens.

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Published on: August 20, 2021

Area of Science:

Urology
Infectious Diseases
Geriatrics

Background:

Diagnosing urinary tract infections (UTIs) in elderly women often requires invasive procedures.
Suprapubic aspiration and bladder catheterization are common but uncomfortable methods for obtaining urine samples.
Accurate urine sample collection is crucial for effective UTI diagnosis and treatment.

Purpose of the Study:

To evaluate a simple, non-invasive method for collecting voided urine samples.
To compare the diagnostic accuracy of this method with suprapubic aspiration for UTI detection in elderly women.
To determine if this technique can differentiate between true infection, asymptomatic bacteriuria, and contamination.

Main Methods:

Urine samples were collected directly into a sterile container during voiding after external cleaning.
Voided samples were compared with simultaneously obtained suprapubic aspirates from the same patients.
Samples were analyzed for pyuria and bacteriuria; Kass criteria and leucocyturia were used for differentiation.

Main Results:

The simple voided urine collection method successfully identified all 13 cases of infection confirmed by suprapubic aspiration.
The technique yielded four false-positive results, indicating a need for careful interpretation.
The method demonstrated potential in differentiating UTI, asymptomatic bacteriuria, and contamination.

Conclusions:

This straightforward urine collection technique is a viable alternative to suprapubic aspiration for diagnosing UTIs in many elderly women.
The method may also eliminate the need for bladder catheterization in this population.
Implementing this simple sampling approach can enhance patient comfort and potentially reduce diagnostic costs.