Changes in expression and localization of hemopexin and its transcripts in injured nervous system: a comparison of...

The recent demonstration of hemopexin synthesis in the adult rat sciatic nerve and its accumulation after injury has raised the question of the possible role of this acute phase protein during the process of nerve repair. To gain insight into its function, we have compared the distribution of both hemopexin and its messenger RNA in the peripheral and the central nervous systems. We find that hemopexin is present in all types of peripheral nerves and ganglia, confined to the extracellular matrix and basement membranes of the endoneurium, blood vessels and connective tissues. After injury, hemopexin messenger RNA is overexpressed by Schwann cells, fibroblasts and invading macrophages. The content in hemopexin protein increases in all nerves studied, without changes in localization. Therefore, hemopexin does not appear to be associated with the fate of myelin or with the regeneration of a particular type of nerve fibre. In the central nervous system, hemopexin messenger RNA cannot be detected and the protein is only found in basement membranes of the vascular system (capillaries, meninges and choroid plexus). Furthermore, hemopexin and its messenger RNA remain absent from the distal part of the injured optic nerves. Our results further support the idea that hemopexin plays specific roles during nerve repair, and that it may be associated with the endoneurial extracellular matrix.