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Related Experiment Videos

Cell cycle checkpoints in bacteria

S Autret1, A Levine, I B Holland

  • 1Institut de Génétique et Microbiologie, URA 2225, Université Paris XI, Orsay, France.

Biochimie
|February 18, 1998
PubMed
Summary
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Bacteria utilize a nutrient-dependent checkpoint to control DNA replication, involving the stringent response and the chromosomal terminator protein (RTP). This system prevents premature initiation and halts replication during starvation, ensuring cell survival.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Bacterial Cell Cycle Regulation

Background:

  • Bacterial DNA replication involves checkpoints to ensure fidelity and survival under stress.
  • The SOS system's SfiA inhibitor blocks cell division upon replication interruption, acting as a dispensable checkpoint.
  • An essential checkpoint signaling replication termination to activate cell division has not been demonstrated.

Purpose of the Study:

  • To investigate the molecular basis of replication fork reactivation in E. coli.
  • To elucidate the nutrient-dependent control system inhibiting DNA replication via the stringent response (RelA/ppGpp).
  • To characterize the role of the chromosomal terminator protein (RTP) in a post-initiation replication checkpoint in B. subtilis.

Main Methods:

Related Experiment Videos

  • Comparative analysis of DNA replication control mechanisms in E. coli and B. subtilis.
  • Investigating the stringent response (RelA/ppGpp) and its effect on DNA replication initiation and progression.
  • Identifying the involvement of the chromosomal terminator protein (RTP) and its binding sites in replication control.

    • Main Results:

    • The stringent response inhibits new DNA replication rounds at oriC in E. coli.
    • In B. subtilis, replication is blocked downstream of oriC, requiring RTP and potentially specific binding sites.
    • A similar post-initiation control mechanism in B. subtilis reversibly blocks replication after premature initiation.

    Conclusions:

    • Bacteria possess a nutrient-dependent replication checkpoint involving the stringent response and RTP.
    • This checkpoint limits replication during starvation and compensates for premature initiation in B. subtilis.
    • The findings reveal a dual-function system crucial for bacterial survival and cell cycle regulation.