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Resuspension-induced ion flux
1New Ideas, Co., Rockville, Maryland 20852, USA.
Abstract:
Pelleting and resuspension of Fura 2-labeled c6 glioma cells leads to a large Ca flux characterized by a high initial internal level of Ca which rapidly declines to close to basal levels. The effect has been termed resuspension-induced ion flux (RIIF). The RIIF effect is temperature dependent and requires external calcium, cytoskeletal integrity, and functional calcium and potassium channels. The magnitude of the RIIF effect is dependent upon pelleting speed, suggesting cell contact and reduction in external fluid medium to be important causative parameters. Several other cell species also exhibit the RIIF effect.
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