Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Targeted disruption of the murine gene coding for the third complement component (C3)

M Pekna1, M A Hietala, T Rosklint

  • 1Department of Medical Biochemistry, University of Göteborg, Sweden.

Scandinavian Journal of Immunology
|February 19, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tamoxifen-independent recombination of reporter genes limits lineage tracing and mosaic analysis using CreER<sup>T2</sup> lines.

Transgenic research·2019
Same author

Adgrf5 contributes to patterning of the endothelial deep layer in retina.

Angiogenesis·2019
Same author

TGF-β1-induced EMT promotes targeted migration of breast cancer cells through the lymphatic system by the activation of CCR7/CCL21-mediated chemotaxis.

Oncogene·2015
Same author

Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish.

Developmental cell·2014
Same author

Short general anaesthesia induces prolonged changes in gene expression in the mouse hippocampus.

Acta anaesthesiologica Scandinavica·2014
Same author

Grafting of neural stem and progenitor cells to the hippocampus of young, irradiated mice causes gliosis and disrupts the granule cell layer.

Cell death & disease·2013

Researchers created mice lacking the third complement component (C3). These mice, unable to activate complement, offer a new model for studying the complement system's role in health and disease.

Area of Science:

  • Immunology
  • Biochemistry

Background:

  • The complement system comprises over 30 plasma and cell membrane proteins.
  • It plays crucial roles in immunity, including inflammation, pathogen elimination, and immune complex clearance.
  • Dysregulated complement activation is implicated in various diseases, such as autoimmune disorders and atherosclerosis.

Purpose of the Study:

  • To generate a novel animal model for investigating the in vivo functions of the complement system.
  • To create mice deficient in the third complement component (C3) to block complement activation early.

Main Methods:

  • Gene targeting was employed to create C3-deficient mice.
  • This deficiency prevents complement activation via all known pathways.

Main Results:

Related Experiment Videos

  • Successfully generated mice lacking the third complement component (C3).
  • These mice serve as a model with inhibited early-stage complement activation.

Conclusions:

  • C3-deficient mice provide a valuable tool for in vivo research on the complement system.
  • This model will aid in understanding complement's role in diverse physiological and pathological contexts.