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Updated: Aug 15, 2026

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue
Published on: April 27, 2012
EM autoradiography of non-sectioned biological material
Abstract:
Methods of EM autoradiography suitable for the study of non-sectioned biological material deposited on a support film from suspension are described. The methods, which include modified procedures for specimen support and emulsion preparation, may also have advantages for the study of any specimen where physical separation of specimen and emulsion is desirable. In a study of the growth of 125I-labelled collagen fibrils reconstituted from solution in vitro, these procedures have allowed the production of EM autoradiographs which show a high resolution and are free from stress artefacts and chemography.

