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Non-peptide inhibitors of HCV serine proteinase
N Kakiuchi1, Y Komoda, K Komoda
1HQL Research Laboratories, Sumitomo Metal Industries, Kyoto, Japan. nkakiuch@virus.kyoto-u.ac.jp
FEBS Letters
|February 19, 1998
Abstract:
We screened a chemical library of 2000 compounds for inhibitors of hepatitis C virus (HCV) serine proteinase using an in vitro screening method measuring the hydrolysis of the peptide substrate. Three compounds were found to be the most potent inhibitors (IC50 < 10(-5) M). Two of them had a similar structure, that of halogenated benzanilide, and were not inhibitory for common serine proteinases. They inhibited the enzyme non-competitively with the substrate. Together with the result of the analogous compounds in the chemical library, the presumed structural requirements of the inhibition are pointed out.