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Adenosine A1 receptor-mediated modulation of dopamine D1 receptors in stably cotransfected fibroblast cells

S Ferré1, M Torvinen, K Antoniou

  • 1Department of Neuroscience, Karolinska Institute, S-171 77 Stockholm, Sweden.

The Journal of Biological Chemistry
|March 21, 1998
PubMed

Insights

Adenosine A1 receptors inhibit dopamine D1 receptors, affecting their binding and signaling. This interaction was observed in cell lines, revealing a key mechanism in receptor modulation.

Area of Science:

  • Neuroscience
  • Molecular Pharmacology

Background:

  • Adenosine A1 receptors and dopamine D1 receptors are crucial G protein-coupled receptors involved in various physiological processes.
  • Understanding the interplay between these receptors is vital for deciphering complex signaling pathways in the central nervous system.

Purpose of the Study:

  • To investigate the antagonistic interactions between adenosine A1 receptors and dopamine D1 receptors.
  • To elucidate the mechanisms underlying this modulation at the receptor binding and signaling levels.

Main Methods:

  • Utilized a mouse Ltk- cell line stably co-transfected with human adenosine A1 and dopamine D1 receptors.
  • Employed receptor binding assays and cAMP accumulation assays to study receptor interactions.
  • Investigated the effects of adenosine A1 receptor agonists and antagonists, as well as pertussis toxin.

Main Results:

  • Adenosine A1 receptor activation decreased the proportion of high-affinity dopamine D1 receptors.
  • Adenosine A1 receptor agonists inhibited dopamine-induced cAMP accumulation in a concentration-dependent manner.
  • Blockade of adenosine A1 receptor signaling increased basal and dopamine-stimulated cAMP levels, indicating tonic A1 receptor activation.

Conclusions:

  • Adenosine A1 receptors antagonistically modulate dopamine D1 receptors.
  • This modulation occurs at both the level of receptor binding affinity and second messenger generation (cAMP).
  • Findings suggest a significant cross-talk between adenosine and dopamine signaling pathways.

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