Bioavailability of ascorbic acid in orange juice
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Updated: Oct 8, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
Abstract:
Several orange juice preparations were analyzed for biologically active and inactive ascorbic acid derivatives --L-ascorbic acid , dehydro-L-ascorbic acid, and inactive 2,3-diketo-L-gulonic acid. Fresh orange juice contained from 4 to 7 mg/100 ml of inactive ascorbic acid. In contrast, pasteurized orange juice contained from 15 to 30 mg/100 ml of inactive ascorbic acid (up to 400% more than fresh orange juice). Samples of fresh orange juice contained as much as 100% more active ascorbic acid than samples of pasteurized orange juice. There is great variability in prepared orange juice derivatives with a substantial decrease in bioavailability of active ascorbic acid.
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