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Related Experiment Videos

Early intrathymic precursor cells acquire a CD4(low) phenotype

A M Michie1, J R Carlyle, J C Zúñiga-Pflücker

  • 1Department of Immunology, University of Toronto, Ontario, Canada.

Journal of Immunology (Baltimore, Md. : 1950)
|February 20, 1998
PubMed
Summary

Early thymic progenitor cells (C4Dlow) can acquire CD4 surface expression passively from surrounding cells. This acquired CD4 phenotype is not an intrinsic characteristic of these progenitor cells.

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Area of Science:

  • Immunology
  • Cell Biology
  • Developmental Biology

Background:

  • C4Dlow cells are the earliest lymphoid progenitor cells in the adult thymus.
  • Thymic progenitors in fetal and RAG-2-deficient mice lack the characteristic CD4 low expression.

Purpose of the Study:

  • To investigate the origin of CD4 low expression on thymic progenitor cells.
  • To determine if CD4 expression is intrinsically present or passively acquired by C4Dlow cells.

Main Methods:

  • Induced differentiation of thymocytes in RAG-2 deficient mice.
  • Analysis of mixed bone marrow chimeras using CD4 deficient and wild-type cells.
  • Acid treatment sensitivity assays.

Main Results:

Related Experiment Videos

  • Differentiation of CD4+ CD8+ thymocytes in RAG-2-/- mice led to CD4 low expression on progenitor cells.
  • Genetically CD4 deficient progenitor cells acquired a CD4 low phenotype in mixed chimeras.
  • CD4 expression on C4Dlow cells was sensitive to acid treatment, suggesting passive acquisition.

    • Conclusions:

    • Low-level CD4 surface expression on thymic progenitor cells is passively acquired from the thymic microenvironment.
    • This acquired phenotype challenges the notion of CD4 as an integral cell surface molecule on these progenitors.
    • Suggests other low-level cell surface molecule expressions may also be acquired phenotypes.