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Related Experiment Videos

Activated polymorphonuclear leukocytes affect red blood cell aggregability

O K Baskurt1, H J Meiselman

  • 1Department of Physiology and Biophysics, University of Southern California School of Medicine, Los Angeles 90033, USA.

Journal of Leukocyte Biology
|February 20, 1998
PubMed
Summary

Activated polymorphonuclear leukocytes (PMN) significantly increase red blood cell (RBC) aggregation and decrease RBC deformability. These effects are mediated by proteolytic enzymes and oxygen free radicals released by activated PMN.

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Area of Science:

  • Hematology
  • Immunology
  • Cell Biology

Background:

  • Leukocytes, particularly polymorphonuclear leukocytes (PMN), can release reactive oxygen species and proteolytic enzymes.
  • Red blood cells (RBC) are susceptible to damage from these mediators when in close proximity to activated leukocytes.

Purpose of the Study:

  • To investigate the impact of activated PMN on RBC aggregability and other RBC properties.
  • To elucidate the mechanisms by which activated PMN influence RBC characteristics.

Main Methods:

  • Human PMN and RBC were isolated and co-incubated.
  • PMN were activated using tumor necrosis factor alpha (TNF-alpha) and N-formyl-methionyl-leucyl-phenylalanine (fMLP).
  • RBC aggregation, deformability, and partition coefficients were measured after incubation; specific inhibitors were used to identify mediating factors.

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Main Results:

  • Activated PMN significantly increased RBC aggregation.
  • RBC deformability and partition coefficients were significantly decreased following PMN activation.
  • The observed effects were mitigated by inhibitors of proteolytic enzymes (phenylmethylsulfonyl fluoride) and reactive oxygen species (superoxide dismutase and catalase).

Conclusions:

  • Activated PMN markedly alter RBC aggregability and deformability.
  • These PMN-mediated effects are attributable to both proteolytic enzymes and oxygen free radicals.
  • PMN-RBC interactions may play a role in enhanced RBC aggregation in clinical conditions and during RBC aging.