Low concentrations of muscarine potentiate M-current in bullfrog sympathetic B-neurones

The concentration-dependence of the effect of muscarine on M-current (IM) and the underlying M-conductance (gM) in B-cells of bullfrog sympathetic ganglion was examined using whole-cell recording techniques. High concentrations of muscarine (> or = 200 nM) produced the classical suppression and over-recovery of steady-state IM at -30 mV. By contrast, low concentrations of muscarine (< or = 30 nM) shifted the gM activation curve to more negative potentials, increased the activation time constant (tau a) and increased steady-state IM. This effect may reflect muscarine-induced changes in submembrane Ca2+ concentration.