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Related Experiment Videos

High throughput liquid chromatography/mass spectrometry bioanalysis using 96-well disk solid phase extraction plate

H Simpson1, A Berthemy, D Buhrman

  • 1Sanofi Pharmaceuticals, Inc., Malvern, PA 19355, USA.

Rapid Communications in Mass Spectrometry : RCM
|February 21, 1998
PubMed
Summary

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Automated 96-well disk plate solid phase extraction (SPE) using robotics enables rapid bioanalysis of drugs and metabolites. This high-throughput method significantly reduces sample preparation time and disposable supply costs for clinical studies.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Pharmacology

Background:

  • Traditional solid phase extraction (SPE) methods can be time-consuming and labor-intensive for high-throughput bioanalysis.
  • The need for efficient sample preparation techniques is critical in supporting large-scale clinical studies for drug and metabolite analysis.

Purpose of the Study:

  • To develop and optimize a 96-well disk plate SPE method for automated sample preparation.
  • To evaluate the performance of robotic automation for high-throughput SPE in LC/MS/MS bioanalysis.
  • To compare the 96-well disk plate SPE approach with traditional SPE methods in terms of efficiency and cost.

Main Methods:

  • Development and characterization of a 96-well disk plate (Empore) for SPE.
  • Modification of a robotic system (MultiProbe) for automated operation of the 96-well plate.

Related Experiment Videos

  • Optimization of SPE parameters for pharmaceutical compounds in biological matrices.
  • Validation of the automated method for drug and metabolite extraction.

    • Main Results:

    • The automated 96-well disk plate SPE system enabled simultaneous extraction of 96 samples, significantly reducing preparation time (1-1.5 hours per batch).
    • The use of disk sorbents allowed for small elution volumes, facilitating a 'dilute and shoot' approach.
    • Considerable savings in disposable supplies were achieved compared to traditional SPE methods.
    • The technology demonstrated successful application in supporting multiple clinical studies.

    Conclusions:

    • Automated 96-well disk plate SPE is a highly efficient and cost-effective technology for high-throughput bioanalysis of drugs and metabolites.
    • This robotic approach streamlines sample preparation, making it suitable for supporting large clinical trials.
    • The 'dilute and shoot' capability simplifies downstream analysis and improves overall workflow efficiency.