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Standardized molecular typing of Candida albicans strains
A Lischewski1, D Harmsen, J Hacker
1Zentrum für Infektionsforschung, Universität Würzburg, Germany.
Mycoses
|February 21, 1998
Abstract:
A method is presented for the standardization of Candida albicans DNA fingerprinting, which is based on Southern hybridization of EcoRI-digested chromosomal DNA with the moderately repetitive DNA element CARE-2 and the subsequent rehybridization of the blots with a molecular size marker also included in each DNA sample. This method resulted in extremely precise alignment of all strain-specific CARE-2 hybridization patterns, even when analysed on different gels, and will enhance the accuracy of genetic relationship determinations in epidemiological studies including large numbers of strains.