Related Experiment Video
Updated: Aug 16, 2026
08:47
Super-resolution Imaging of the Bacterial Division Machinery
Published on: January 21, 2013
Abstract:
A method for rapid measurement of the resolution of a microscope by finding the smallest distance between pairs of points that appear on two successive micrographs is described. The method based on the visualization of moire pattern gives the same result as other tests with a precision sufficient for routine checks. Neither a special instrument nor time consuming training are required.
More Related Videos
Related Concept Videos
01:18Imaging Biological Samples with Optical Microscopy
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
01:37Super-resolution Fluorescence Microscopy
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.