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A highly conserved region upstream of the fibronectin alternative exon EIIIA 3' splice site interacts with
P A Norton1, T Uporova, V D Bennett
1Department of Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA.
Biochimica Et Biophysica Acta
|February 25, 1998
Abstract:
Sequencing of chicken fibronectin genomic DNA and interspecies sequence comparisons reveal a highly conserved region upstream of the alternatively spliced exon EIIIA. UV-crosslinking of RNAs corresponding to this region from the chicken and rat genes with HeLa nuclear extract demonstrates that both RNAs interact with similar proteins. However, both RNAs crosslink to a 70 kDa protein present in nuclear extracts from cells and tissues that include exon EIIIA, but not in extracts from tissues that exclude the exon. This protein represents a candidate cell-type-specific factor involved in exon EIIIA inclusion.