Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hepatic microsomal induction and hepatic transport

E Fischer, Z Gregus, F Varga

    Acta Medica Academiae Scientiarum Hungaricae
    |January 1, 1979
    PubMed
    Summary

    Phenobarbital pretreatment in rats enhances the liver

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Tumour of the choroid with yellowish subretinal deposits].

    Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·2003
    Same author

    Hand-assisted laparoscopic splenectomy for giant spleens.

    Surgical endoscopy·2003
    Same author

    Coronary artery bypass grafting supported with intracardiac microaxial pumps versus normothermic cardiopulmonary bypass: a prospective randomized trial.

    European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery·2002
    Same author

    Inhibition of fusion of Chlamydia trachomatis inclusions at 32 degrees C correlates with restricted export of IncA.

    Infection and immunity·2002
    Same author

    One-year survival and neurological outcome after pediatric cardiopulmonary resuscitation.

    Intensive care medicine·2002
    Same author

    Parathyroid hormone-related protein and its receptors: nuclear functions and roles in the renal and cardiovascular systems, the placental trophoblasts and the pancreatic islets.

    British journal of pharmacology·2001

    Area of Science:

    • Pharmacology and Toxicology
    • Hepatology
    • Drug Metabolism

    Background:

    • Hepatic microsomal enzymes play a crucial role in drug metabolism and xenobiotic elimination.
    • Understanding how drug-induced changes in liver function affect the transport and excretion of organic anions is vital for drug development and safety.
    • Barbiturates are known to induce hepatic microsomal enzymes, but their impact on biliary excretion pathways requires further elucidation.

    Purpose of the Study:

    • To investigate the effects of phenobarbital pretreatment on hepatic microsomal enzyme induction and the biliary excretion of various organic anions in rats.
    • To explore the relationship between barbiturate-induced microsomal enzyme induction and alterations in hepatic transport and excretion of exogenous organic anions.
    • To determine if the enhanced biliary excretion of organic anions by barbiturates is dependent on microsomal enzyme induction.

    Main Methods:

    • Rats were pretreated with phenobarbital (75 mg/kg daily for 5 days) or other barbiturates.
    • Measurements included hepatic microsomal enzyme activity (hexobarbital sleeping time, cytochrome P-450, microsomal protein), liver weight, and hepatic uptake, biotransformation, and biliary excretion of model organic anions (e.g., bromcresol green, BSP-GSH, amaranth).
    • Concentrations of organic anions in the liver were also assessed.

    Main Results:

    • Phenobarbital pretreatment significantly induced hepatic microsomal enzymes and enhanced the biliary excretion of most tested organic anions, except indocyanine green.
    • Biotransformation of bromosulfthalein (BSP) with glutathione was increased by phenobarbital.
    • Other barbiturates (barbital, butobarbital) also induced microsomal enzymes and stimulated biliary excretion, while pentobarbital and amobarbital were ineffective in stimulating excretion despite inducing enzymes.

    Conclusions:

    • Phenobarbital pretreatment in rats leads to enhanced biliary excretion of several exogenous organic anions, independent of changes in their hepatic concentrations.
    • The ability of barbiturates to stimulate biliary excretion of organic anions appears to be independent of their capacity for microsomal enzyme induction.
    • These findings suggest distinct mechanisms underlying barbiturate-induced microsomal enzyme induction and the modulation of hepatic transport systems for organic anions.

    Related Experiment Videos