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Increase in the helper inducer (CD4+CD29+) T lymphocytes in smokers
T Tanigawa1, S Araki, A Nakata
1Department of Public Health, Faculty of Medicine, University of Tokyo, Japan.
Industrial Health
|February 25, 1998
Summary
Smoking significantly increases CD4+CD29+ helper inducer T lymphocytes in male smokers. This specific T lymphocyte subset increase is responsible for the elevated total CD4+ T cells observed in smokers.
Area of Science:
- Immunology
- Hematology
Background:
- Previous research indicated elevated CD4+ T lymphocytes in smokers' peripheral blood.
- Understanding specific T lymphocyte subpopulations affected by smoking is crucial for immune response insights.
Purpose of the Study:
- To investigate the impact of smoking on various T lymphocyte subpopulations, including CD4+ T cells.
- To identify specific CD4+ T lymphocyte subsets that contribute to the observed increase in total CD4+ T cells in smokers.
Main Methods:
- Two-color flow cytometry using anti-2H4 (CD45RA) and anti-4B4 (CD29) monoclonal antibodies.
- Analysis of CD4+ T lymphocytes, CD8+ T lymphocytes, total T lymphocytes (CD3+), B lymphocytes (CD19+), and natural killer cells (CD16+).
- Comparison between 8 male smokers and 22 age-matched male non-smokers.
Main Results:
- Smokers exhibited a significantly higher number of CD4+CD29+ T lymphocytes compared to non-smokers.
- The total count of CD4+ T lymphocytes was also significantly elevated in smokers.
- No significant differences were observed in CD8+ T, CD19+ B, or CD16+ NK cell counts between the groups.
Conclusions:
- The increase in CD4+CD29+ (helper inducer) T lymphocytes is identified as the primary driver for the elevated total CD4+ T lymphocytes in smokers.
- Smoking appears to selectively impact specific T lymphocyte subsets, particularly the helper inducer population.