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Improved method for measuring tartrate-resistant acid phosphatase activity in serum
1Department of Clinical Pathology, Hyogo College of Medicine, Japan.
Clinical Chemistry
|February 25, 1998
Summary
This study presents a new method to specifically measure osteoclast-derived tartrate-resistant acid phosphatase (TrACP) in serum using fluoride sensitivity. This improved assay offers accurate kinetic measurements for various age groups.
Area of Science:
- Biochemistry
- Enzymology
- Clinical Chemistry
Background:
- Tartrate-resistant acid phosphatase (TrACP) is crucial in bone resorption.
- Existing methods lack specificity for osteoclast-derived TrACP.
- Distinguishing TrACP sources (erythrocytes, platelets, macrophages) is vital.
Purpose of the Study:
- To develop an improved kinetic assay for specific measurement of osteoclast-derived TrACP in serum.
- To differentiate skeletal TrACP from other TrACP isoforms based on fluoride sensitivity.
Main Methods:
- Utilized 2,6-dichloro-4-acetylphenyl phosphate as a substrate at pH 6.2.
- Employed fluoride sensitivity to specifically target osteoclast-derived TrACP (TrFsACP).
- Incorporated hexadimethrine bromide (Polybrene) to enhance TrFsACP activity in serum.
Main Results:
- The developed method accurately measures TrFsACP activity, unaffected by hemolysis up to 0.9 g/L hemoglobin.
- Mean TrFsACP activity in young adults: 20.4 +/- 2.8 U/L (men) and 16.4 +/- 2.3 U/L (women).
- Highest TrFsACP activity observed in children (<15 years), followed by elderly subjects (>60 years).
Conclusions:
- This novel assay enables specific kinetic measurement of osteoclast-derived TrACP in serum.
- The method's robustness and specificity make it valuable for clinical and research applications.
- Age-related variations in TrFsACP activity highlight its potential as a biomarker.